KHK Polyklonaler Antikörper

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• KD/KO Validated

KHK Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 15681-1-AP

Synonyme

Hepatic fructokinase, Ketohexokinase, ketohexokinase (fructokinase), KHK

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Galerie der Validierungsdaten

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  WB analysis using 15681-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15681-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15681-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15681-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15681-1-AP

  IP Result of anti-KHK (IP:15681-1-AP, 4ug; Detection:15681-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IP experiment of HepG2 using 15681-1-AP

  IP result of anti-KHK(IP:15681-1-AP, 4ug; Detection:15681-1-AP 1:1000) with HepG2 cells lysate 960 ug.

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  IHC staining of human liver using 15681-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15681-1-AP (KHK Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 15681-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15681-1-AP (KHK Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human liver using 15681-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15681-1-AP (KHK Antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human liver using 15681-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15681-1-AP (KHK Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 15681-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 15681-1-AP (KHK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15681-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using KHK antibody (15681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, humanes Lebergewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below

Produktinformation

15681-1-AP bindet in WB, IP, IHC, IF, ELISA KHK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KHK fusion protein Ag8182
• Vollständiger Name: ketohexokinase (fructokinase)
• Berechnetes Molekulargewicht: 298 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33-35 kDa
• GenBank-Zugangsnummer: BC006233
• Gene symbol: KHK
• Gene ID (NCBI): 3795
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ketohexokinase catalyzes conversion of fructose to fructose-1-phosphate, it is the first enzyme with a specialized pathway that catabolizes dietary fructose. Fructose metabolism seems to provide cancer cells with the supplementary fuel required for proliferation and metastasis.

Publikationen

Species	Application	Title
mouse	WB,IHC	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
human	WB	Adv Sci (Weinh); NAT10/ac4C/FOXP1 Promotes Malignant Progression and Facilitates Immunosuppression by Reprogramming Glycolytic Metabolism in Cervical Cancer; Authors - Xiaona Chen
human	WB	Brain; C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.; Authors - Scott P Allen
mouse	WB	Endocrinology; Hepatic but not intestinal FBP1 is required for fructose metabolism and tolerance; Authors - Qiufang Bai
human	WB	Biochem Biophys Res Commun; Ketohexokinase is involved in fructose utilization and promotes tumor progression in glioma.; Authors - Wenbo Gao
human	WB	PeerJ; Increased utilization of fructose has a positive effect on the development of breast cancer.; Authors - Xiajing Fan ; KD Validated