KEPI Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KEPI Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24933-1-AP

Synonyme

CPI17 like, KEPI, Kinase enhanced PP1 inhibitor, NY BR 81, PPP1R14C

---

Galerie der Validierungsdaten

• 

  WB analysis using 24933-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24933-1-AP (KEPI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24933-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24933-1-AP (KEPI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24933-1-AP

  WB result of KEPI antibody (24933-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KEPI transfected HeLa cells.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, MDA-MB-231-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24933-1-AP bindet in WB, ELISA KEPI und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KEPI fusion protein Ag20645
• Vollständiger Name: protein phosphatase 1, regulatory (inhibitor) subunit 14C
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 20-22 kDa
• GenBank-Zugangsnummer: BC015773
• Gene symbol: PPP1R14C
• Gene ID (NCBI): 81706
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP1R14C, found as a morphine‐regulated brain gene, encodes protein phosphatase 1 regulatory inhibitor subunit 14C, and is identified as a potent inhibitor of PP1. Horvath and colleagues reported that PPP1R14C inhibited the release of neurotransmitters and promoted neuronal exocytosis by retaining synaptosome associated protein 25 (SNAP25) at a phosphorylated state. Moreover, overexpression of PPP1R14C increased the phosphorylation of RB transcriptional co‐repressor 1 (RB1) to protect leukemic cells from chemotherapy.