RBP2 Polyklonaler Antikörper

RBP2 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 18825-1-AP

Synonyme

Histone demethylase JARID1A, JARID1A, KDM5A, lysine (K) demethylase 5A, Lysine specific demethylase 5A, RBBP 2, RBBP2, RBP2

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 18825-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18825-1-AP (RBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 18825-1-AP

  IP result of anti-RBP2(IP:18825-1-AP, 4ug; Detection:18825-1-AP 1:500) with HeLa cells lysate 1280 ug.

• 

  IF Staining of HeLa using 18825-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18825-1-AP (RBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

18825-1-AP bindet in WB, IP, IF, ELISA RBP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: lysine (K)-specific demethylase 5A
• Berechnetes Molekulargewicht: 192 kDa
• Beobachtetes Molekulargewicht: 192 kDa
• GenBank-Zugangsnummer: NM_005056
• Gene symbol: KDM5A
• Gene ID (NCBI): 5927
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KDM5A, also named as JARID1A, RBBP2 and RBP2, belongs to the JARID1 histone demethylase family. KDM5A is a Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. RBP2 may stimulate transcription mediated by nuclear receptors. It may be involved in transcriptional regulation of Hox proteins during cell differentiation. It may participate in transcriptional repression of cytokines such as CXCL12.

Publikationen

Species	Application	Title
mouse	WB,IF	Environ Toxicol; JMJD3 downregulates IL4i1 aggravating lipopolysaccharide-induced acute lung injury via H3K27 and H3K4 demethylation; Authors - Yizhuo Gao
human	WB	Int J Biol Sci; KDM5B demethylates H3K4 to recruit XRCC1 and promote chemoresistance.; Authors - Wenxia Xu
rat	IF	Life Sci; Treadmill exercise improves neurological function by inhibiting autophagy and the binding of HMGB1 to Beclin1 in MCAO juvenile rats.; Authors - Guoyuan Pan