KDM1B Monoklonaler Antikörper

KDM1B Monoklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E1A3

Kat-Nr. : 67886-1-Ig

Synonyme

AOF1, bA204B7.3, C6orf193, dJ298J15.2, DKFZp686I0412, FLJ33898, FLJ34109, FLJ43328, KDM1B, LSD2

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Galerie der Validierungsdaten

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  WB analysis using 67886-1-Ig

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 67886-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 67886-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67886-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of SW 1990 using 67886-1-Ig

  1X10^6 SW 1990 cells were intracellularly stained with 0.4 ug Anti-HUMAN KDM1B (67886-1-Ig, Clone:1E1A3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, A549-Zellen, C6-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in FC: SW 1990-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67886-1-Ig bindet in WB, FC, ELISA KDM1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KDM1B fusion protein Ag30672
• Vollständiger Name: amine oxidase (flavin containing) domain 1
• Berechnetes Molekulargewicht: 92 kDa
• Beobachtetes Molekulargewicht: 92 kDa
• GenBank-Zugangsnummer: NM_001364614
• Gene symbol: AOF1
• Gene ID (NCBI): 221656
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.