KCTD2/5/17 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KCTD2/5/17 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 15553-1-AP

Synonyme

KCTD17, KCTD2, KCTD5

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 15553-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15553-1-AP (KCTD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15553-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15553-1-AP (KCTD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15553-1-AP

  IP Result of anti-KCTD5 (IP:15553-1-AP, 4ug; Detection:15553-1-AP 1:500) with HeLa cells lysate 1200ug.

• 

  IHC staining of human ovary tumor using 15553-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15553-1-AP (KCTD5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 15553-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15553-1-AP (KCTD5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 15553-1-AP

  Immunofluorescent analysis of Hela cells, using KCTD5 antibody 15553-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 15553-1-AP

  1X10^6 HeLa cells were stained with 0.2ug KCTD5 antibody (15553-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IP: See 1 publications below

Produktinformation

15553-1-AP bindet in WB, IP, IHC, IF, FC, ELISA KCTD2/5/17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCTD2/5/17 fusion protein Ag7915
• Vollständiger Name: potassium channel tetramerisation domain containing 5
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC007314
• Gene symbol: KCTD5
• Gene ID (NCBI): 54442
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KCTD5 belongs to the human potassium (K+) channel tetramerization domain (KCTD) family of proteins which share sequence similarity with the cytoplasmic domain of voltage-gated K+ channels (Kv channels). The KCTD proteins have relatively conserved N-terminal domains and variable C-termini (PMID: 24268103). KCTD5 interacts with cullin3 and has been proposed to function as substrate adapter for cullin3 based ubiquitin E3 ligases (PMID: 26188516). This polyclonal antibody raised against full-length recombinant protein of human KCTD5 (26 kDa) can cross-react with other KCTD proteins, including KCTD2 (29 kDa), KCTD17 (33-36 kDa).

Publikationen

Species	Application	Title
human	WB	Sci Adv; Structural basis for the ubiquitination of G protein βγ subunits by KCTD5/Cullin3 E3 ligase; Authors - Wentong Jiang
human	WB	Nat Commun; Knowledge graph-based recommendation framework identifies drivers of resistance in EGFR mutant non-small cell lung cancer.; Authors - Anna Gogleva ; KO Validated
human	WB	Diabetes; HOXC10 Suppresses Browning to Maintain White Adipocyte Identity.; Authors - H Y Angeline Tan
human	WB	Elife; Genome-wide CRISPR screening reveals genetic modifiers of mutant EGFR dependence in human NSCLC.; Authors - Hao Zeng
rat	WB	PLoS Genet; Conserved properties of Drosophila Insomniac link sleep regulation and synaptic function.; Authors - Qiuling Li
human	WB,IP	J Proteome Res; Human Multisubunit E3 Ubiquitin Ligase Required for Heterotrimeric G-Protein β-Subunit Ubiquitination and Downstream Signaling.; Authors - Brian D Young ; KD Validated