KCTD10 Polyklonaler Antikörper

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• KD/KO Validated

KCTD10 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 27279-1-AP

Synonyme

KCTD10, MSTP028, ULR061, ULRO61

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Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 27279-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 27279-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 27279-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 27279-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 27279-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 27279-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27279-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below

Produktinformation

27279-1-AP bindet in WB, ELISA KCTD10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCTD10 fusion protein Ag25971
• Vollständiger Name: potassium channel tetramerisation domain containing 10
• Berechnetes Molekulargewicht: 313 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC040062
• Gene symbol: KCTD10
• Gene ID (NCBI): 83892
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Unbiased interrogation of functional lysine residues in human proteome; Authors - Ying Bao
human	WB	Nat Commun; Regulation of TRIF-mediated innate immune response by K27-linked polyubiquitination and deubiquitination.; Authors - Xin Wu ; KO Validated
mouse	WB	Front Cell Dev Biol; Cullin3-TNFAIP1 E3 Ligase Controls Inflammatory Response in Hepatocellular Carcinoma Cells via Ubiquitination of RhoB.; Authors - Yue Liu ; KD Validated
human,rat	WB	Genet Res (Camb); Inhibition of KCTD10 Affects Diabetic Retinopathy Progression by Reducing VEGF and Affecting Angiogenesis; Authors - Yun Feng ; KD Validated