GIRK2 Polyklonaler Antikörper

GIRK2 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 21647-1-AP

Synonyme

BIR1, GIRK 2, GIRK2, hiGIRK2, KATP 2, KATP2, KCNJ6, KCNJ7, KIR3.2

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Galerie der Validierungsdaten

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  WB analysis using 21647-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21647-1-AP (GIRK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21647-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21647-1-AP (GIRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 21647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21647-1-AP (GIRK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21647-1-AP (GIRK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

21647-1-AP bindet in WB, IHC, IF, ELISA GIRK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GIRK2 fusion protein Ag16344
• Vollständiger Name: potassium inwardly-rectifying channel, subfamily J, member 6
• Berechnetes Molekulargewicht: 423 aa, 48 kDa
• Beobachtetes Molekulargewicht: 45-48 kDa
• GenBank-Zugangsnummer: BC101547
• Gene symbol: KCNJ6
• Gene ID (NCBI): 3763
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GIRK2 (also known as Kir3.2), encoded by KCNJ6 gene, is a member of the G protein-coupled inwardly-rectifying potassium channel (GIRK, Kir3) family of inward rectifier potassium channels. GIRK channels are activated following stimulation of G protein-coupled receptors (PMID: 26422984). They play important roles in regulating cellular excitabilities in the heart and brain (PMID: 31043612). Mutations in KCNJ6 gene are associated with Keppen-Lubinsky Syndrome (PMID: 25620207).

Publikationen

Species	Application	Title
mouse	WB	Mol Ther Nucleic Acids; Modulation of miR-181 influences dopaminergic neuronal degeneration in a mouse model of Parkinson's disease.; Authors - Colleen S Stein
mouse	WB	FASEB J; Electro-acupuncture alleviates adolescent cocaine exposure-enhanced anxiety-like behaviors in adult mice by attenuating the activities of PV interneurons in PrL.; Authors - Jiaxun Nie
human	IF	Front Neurosci; Deregulation of mTORC1-TFEB axis in human iPSC model of GBA1-associated Parkinson's disease; Authors - Fahad Mubariz
		Prog Neurobiol; TRPM2 as a conserved gatekeeper determines the vulnerability of DA neurons by mediating ROS sensing and calcium dyshomeostasis; Authors - Peiwu Ye