KCNJ15 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KCNJ15 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15988-1-AP

Synonyme

IRKK, KCNJ14, KCNJ15, KIR1.3, KIR4.2

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 15988-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15988-1-AP (KCNJ15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15988-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15988-1-AP (KCNJ15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15988-1-AP

  IP Result of anti-KCNJ15 (IP:15988-1-AP, 4ug; Detection:15988-1-AP 1:500) with HepG2 cells lysate 1000ug.

• 

  IHC staining of human liver using 15988-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15988-1-AP (KCNJ15 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15988-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15988-1-AP (KCNJ15 antibody) at dilution of 1:100 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

15988-1-AP bindet in WB, IP, IHC, IF, ELISA KCNJ15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCNJ15 fusion protein Ag8795
• Vollständiger Name: potassium inwardly-rectifying channel, subfamily J, member 15
• Berechnetes Molekulargewicht: 375 aa, 43 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC013327
• Gene symbol: KCNJ15
• Gene ID (NCBI): 3772
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KCNJ15, also named as KIR1.3, KIR4.2, and IRKK, is a multi-pass membrane protein belonging to the inwardly-rectifying potassium channel (KIR) family. KIR channels perform functions as diverse as the regulation of resting membrane potential, maintenance of potassium ions homeostasis, control of heart rate and hormone secretion. Cloned from human kidney, the KCNJ15 gene is localized on chromosome 21 in the Down syndrome chromosome region 1 (DCR1) and has been identified as a type 2 diabetes-associated risk gene. The gene is most readily expressed in the pancreas and kidney and less in the lung. (PMID: 8995301; 9299242; 20085713)

Publikationen

Species	Application	Title
human	WB, IF	Nat Commun; KCNJ15/Kir4.2 couples with polyamines to sense weak extracellular electric fields in galvanotaxis.; Authors - Ken-Ichi Nakajima ; KD Validated
mouse	IHC	DNA Res; Identification of novel genes selectively expressed in the follicle-associated epithelium from the meta-analysis of transcriptomics data from multiple mouse cell and tissue populations.; Authors - Kobayashi Atsushi A
human	IHC	Ann Surg Oncol; KCNJ15 Expression and Malignant Behavior of Esophageal Squamous Cell Carcinoma.; Authors - Shunsuke Nakamura