KCNJ1 Polyklonaler Antikörper

KCNJ1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20953-1-AP

Synonyme

KCNJ1, KIR1.1, ROMK, ROMK1

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 20953-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20953-1-AP (KCNJ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20953-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20953-1-AP (KCNJ1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20953-1-AP

  IP Result of anti-KCNJ1 (IP:20953-1-AP, 4ug; Detection:20953-1-AP 1:300) with mouse brain tissue lysate 3600ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

20953-1-AP bindet in WB, IP, IF, ELISA KCNJ1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCNJ1 fusion protein Ag15100
• Vollständiger Name: potassium inwardly-rectifying channel, subfamily J, member 1
• Berechnetes Molekulargewicht: 391 aa, 45 kDa
• Beobachtetes Molekulargewicht: 75-80 kDa
• GenBank-Zugangsnummer: BC074752
• Gene symbol: KCNJ1
• Gene ID (NCBI): 3758
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KCNJ1, also called ROMK, is an inward-rectifying K+ channel responsible for basal (non-flow stimulated) K+ secretion. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium. This channel is activated by internal ATP and can be blocked by external barium. The calculated molecule weight is 45kDa, 20953-1-AP can detect the band around 75-80 kDa, it has been considered to be a dimer of KCNJ1.(PMID: 17804670; 7929082; 15767585)

Publikationen

Species	Application	Title
mouse	IF	J Am Soc Nephrol; Activation of mTORC1 in Collecting Ducts Causes Hyperkalemia.; Authors - Zhenguo Chen
		Am J Physiol Renal Physiol; (Pro)Renin Receptor Regulates Potassium Homeostasis through a Local Mechanism.; Authors - Chuanming Xu