Kv4.2 Polyklonaler Antikörper

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• KD/KO Validated

Kv4.2 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 21298-1-AP

Synonyme

KCND2, KIAA1044, Kv4.2, RK5

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Galerie der Validierungsdaten

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  WB analysis of A549 using 21298-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21298-1-AP (Kv4.2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21298-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21298-1-AP (Kv4.2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 21298-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 21298-1-AP (Kv4.2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 21298-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21298-1-AP (Kv4.2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21298-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21298-1-AP (Kv4.2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 21298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 21298-1-AP (Kv4.2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 21298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 21298-1-AP (Kv4.2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, DU 145-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

21298-1-AP bindet in WB, IHC, IF, ELISA Kv4.2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Kv4.2 fusion protein Ag15879
• Vollständiger Name: potassium voltage-gated channel, Shal-related subfamily, member 2
• Berechnetes Molekulargewicht: 630 aa, 71 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC110449
• Gene symbol: KCND2
• Gene ID (NCBI): 3751
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Voltage-gated potassium or Kv channels, specifically those mediating low threshold, rapidly inactivating Ito and IA currents, are known to regulate cardiac and neuronal membrane excitability, respectively (PMID: 12829703). Voltage-gated potassium channel subunit Kv4.2, encoded by the KCND2 gene, belongs to the potassium channel family and D (Shal) subfamily. It is a pore-forming alpha subunit of voltage-gated rapidly inactivating A-type potassium channels. Kv4.2 is highly expressed in the brain (PMID: 10551270). It is a major constituent of A-type potassium currents and a key regulator of neuronal membrane excitability (PMID: 22539834).

Publikationen

Species	Application	Title
mouse		Neurobiol Dis; MicroRNA inhibition upregulates hippocampal A-type potassium current and reduces seizure frequency in a mouse model of epilepsy.; Authors - Durgesh Tiwari
mouse	WB	Sci Rep; Mir324 knockout regulates the structure of dendritic spines and impairs hippocampal long-term potentiation; Authors - Emma V Parkins
mouse	WB	eNeuro; Estradiol- and Progesterone-Associated Changes in microRNA-Induced Silencing and Reduced Antiseizure Efficacy of an Antagomir in Female Mice; Authors - Durgesh Tiwari
rat	WB,IHC,IF	JCI Insight; Indoxyl sulfate reduces Ito,f by activating ROS/MAPK and NF-κB signaling pathways.; Authors - Jing Yang
rat	WB	Biochem Biophys Res Commun; Pinocembrin alleviates the susceptibility to atrial fibrillation in isoproterenol-induced rats; Authors - Zhangchi Liu
mouse	WB	Res Sq; Age-dependent regulation of dendritic spine density and protein expression in Mir324 KO mice; Authors - Emma V Parkins