KCC4/SLC12A7 Polyklonaler Antikörper

KCC4/SLC12A7 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 26590-1-AP

Synonyme

K Cl cotransporter 4, KCC4, KCC4/SLC12A7, SLC12A7

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Galerie der Validierungsdaten

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  WB analysis using 26590-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26590-1-AP (KCC4/SLC12A7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26590-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 26590-1-AP (KCC4/SLC12A7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BGC-823-Zellen, NCI-H1299-Zellen, Raji-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26590-1-AP bindet in WB, ELISA KCC4/SLC12A7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCC4/SLC12A7 fusion protein Ag24954
• Vollständiger Name: solute carrier family 12 (potassium/chloride transporters), member 7
• Beobachtetes Molekulargewicht: 119 kDa
• GenBank-Zugangsnummer: BC098390
• Gene symbol: SLC12A7
• Gene ID (NCBI): 10723
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KCC4, also named as Solute carrier family 12 member 7(SLC12A7), is a 1083 amino-acid protein, which belongs to the SLC12A transporter family. As a multi-pass membrane protein, the molecular weight of KCC4 is 119 kDa. KCC4 mediates electroneutral potassium-chloride cotransport when activated by cell swelling. Moreover, KCC4 may mediate K+ uptake into Deiters' cells in the cochlea and contribute to K+ recycling in the inner ear. KCC4 is important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti(Uniprot, GeneID:10723).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Bishwa Ranjan (Verified Customer) (08-03-2023)

In the Western blots there is an additional band below at 98kD which seems like a degradation or non-specific binding of the antibody. The bands showing at 120-130kD are accurate as we have seen in the lanes from knockdowns and overexpression of protein KCC4 For reference information about the lanes: 1. MDA KCC4 overexpression 2. MDA KCC4 knockdown sequence1 3. MDA KCC4 knockdown sequence2 4. MDA KCC4 knockdown sequence3 5. MDA scrambled control As you can see in lane 5 which is the control has expression of kCC4 at 120-130 range and for the knockdowns there is a decrease more significantly for 2nd sequence and for overexpression in lane 1 it is quite high so we believe the top band is the expected one and the bottom one is a result of degradation maybe so please advice on this matter. As for the antibody we received it in April 2023 and it is stored at -20 as mentioned.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: MDA-MB-231, A-431

KCC4/SLC12A7 Antibody Western Blot validation (1:2000 dilution) in MDA-MB-231, A-431 (Cat no:26590-1-AP)