KAT2A/GCN5 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

KAT2A/GCN5 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

CloneNo.

1F6G7

Kat-Nr. : 66575-1-Ig

Synonyme

GCN5, GCN5L2, hGCN5, Histone acetyltransferase GCN5, HsGCN5, K(lysine) acetyltransferase 2A, KAT2A, KAT2A/GNC5, Lysine acetyltransferase 2A, PCAF b, STAF97

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Galerie der Validierungsdaten

• 

  WB analysis using 66575-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66575-1-Ig (KAT2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66575-1-Ig

  WB result of KAT2A/GNC5 antibody (66575-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KAT2A/GNC5 transfected MCF-7 cells.

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  WB analysis of HSC-T6 using 66575-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66575-1-Ig (KAT2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66575-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66575-1-Ig (KAT2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

66575-1-Ig bindet in WB, IF, ELISA KAT2A/GCN5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KAT2A/GCN5 fusion protein Ag6940
• Vollständiger Name: K(lysine) acetyltransferase 2A
• Berechnetes Molekulargewicht: 94 kDa
• Beobachtetes Molekulargewicht: 94 kDa
• GenBank-Zugangsnummer: BC032743
• Gene symbol: KAT2A
• Gene ID (NCBI): 2648
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.

Publikationen

Species	Application	Title
mouse	WB	Brain Behav Immun; Kynurenine pathway is altered in BDNF Val66Met knock-in mice: Effect of physical exercise.; Authors - Alessandro Ieraci
mouse	WB	J Bone Miner Res; Inhibition of ACLY Leads to Suppression of Osteoclast Differentiation and Function Via Regulation of Histone Acetylation.; Authors - Qian Guo
human	WB	Development; Dynamic profiling and functional interpretation of histone Kcr and Kla during neural development.; Authors - Shang-Kun Dai ; KD Validated
human	WB,IF	PLoS One; Psychostimulants and opioids differentially influence the epigenetic modification of histone acetyltransferase and histone deacetylase in astrocytes.; Authors - Mayur Doke
human	WB	BMC Cancer; Histone regulator KAT2A acts as a potential biomarker related to tumor microenvironment and prognosis of diffuse large B cell lymphoma; Authors - Zhuoya Yu ; KD Validated