KALRN Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KALRN Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 19740-1-AP

Synonyme

DUET, duo, HAPIP, Kalirin, kalirin, RhoGEF kinase, KALRN, Protein Duo, TRAD

---

Galerie der Validierungsdaten

• 

  WB analysis using 19740-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19740-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 19740-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 19740-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19740-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 19740-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19740-1-AP (KALRN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19740-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19740-1-AP (KALRN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 19740-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 19740-1-AP (KALRN antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, humanes Hirngewebe, humanes Nierengewebe, Ratten-Kolongewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

19740-1-AP bindet in WB, IHC, IF, ELISA KALRN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: kalirin, RhoGEF kinase
• Berechnetes Molekulargewicht: 340 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: NM_007064
• Gene symbol: KALRN
• Gene ID (NCBI): 8997
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KALRN, also named as DUET, DUO, HAPIP and TRAD, belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. KALRN promotes the exchange of GDP by GTP. KALRN activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. KALRN induces lamellipodia independent of its GEF activity. The antibody can recognize isoform1 and isoform2 of KALRN.

Publikationen

Species	Application	Title
mouse	WB	FEBS Lett; Kalirin mediates Rac1 activation downstream of calcium/calmodulin-dependent protein kinase II to stimulate glucose uptake during muscle contraction.; Authors - Sasa Liu
mouse	WB	Biochem Biophys Res Commun; CaMKII and Kalirin, a Rac1-GEF, regulate Akt phosphorylation involved in contraction-induced glucose uptake in skeletal muscle cells.; Authors - Sasa Liu
human	WB,IF	BMC Womens Health; Metabolism-associated molecular classification of cervical cancer; Authors - Min Zhao ; KD Validated
rat	WB	Mol Cell Neurosci; Role and mechanism of EphB3 in epileptic seizures and epileptogenesis through Kalirin; Authors - Hao Huang