JPH1 Polyklonaler Antikörper

JPH1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25979-1-AP

Synonyme

JP 1, JP1, JPH1, junctophilin 1, Junctophilin type 1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 25979-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25979-1-AP (JPH1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25979-1-AP (JPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 25979-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25979-1-AP (JPH1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 25979-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25979-1-AP (JPH1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin using 25979-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 25979-1-AP (JPH1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 25979-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 25979-1-AP (JPH1 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25979-1-AP bindet in WB, IHC,ELISA JPH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JPH1 fusion protein Ag23270
• Vollständiger Name: junctophilin 1
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC140875
• Gene symbol: JPH1
• Gene ID (NCBI): 56704
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Junctophilin-1 is a protein that in humans is encoded by the JPH1 gene. This gene is a member of the junctophilin gene family. Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane.