JMJD6 Polyklonaler Antikörper

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• KD/KO Validated

JMJD6 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 16476-1-AP

Synonyme

JMJD6, jumonji domain containing 6, KIAA0585, Lysyl hydroxylase JMJD6, Phosphatidylserine receptor, Protein PTDSR, PSR, PTDSR, PTDSR1

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 16476-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16476-1-AP (JMJD6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 16476-1-AP

  IP Result of anti-JMJD6 (IP:16476-1-AP, 4ug; Detection:16476-1-AP 1:500) with K-562 cells lysate 1200ug.

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  IHC staining of mouse heart using 16476-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16476-1-AP (JMJD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16476-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16476-1-AP (JMJD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 16476-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16476-1-AP (JMJD6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16476-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16476-1-AP (JMJD6 antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of K-562 using 16476-1-AP

  1X10^6 K-562 cells were stained with 0.2ug JMJD6 antibody (16476-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 3 publications below

Produktinformation

16476-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA JMJD6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JMJD6 fusion protein Ag9590
• Vollständiger Name: jumonji domain containing 6
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC066654
• Gene symbol: JMJD6
• Gene ID (NCBI): 23210
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB,IP,ChIP	Mol Cell; JMJD6 Licenses ERα-Dependent Enhancer and Coding Gene Activation by Modulating the Recruitment of the CARM1/MED12 Co-activator Complex.; Authors - Wei-Wei Gao ; KD Validated
human	WB	PLoS Pathog; JMJD6 negatively regulates cytosolic RNA induced antiviral signaling by recruiting RNF5 to promote activated IRF3 K48 ubiquitination.; Authors - Wei Zhang ; KO Validated
human	WB	J Med Chem; Discovery of Small-Molecule Degraders of the CDK9-Cyclin T1 Complex for Targeting Transcriptional Addiction in Prostate Cancer; Authors - Jiacheng Li
human	ChIP	Cell Biol Int; c-Jun-mediated JMJD6 restoration enhances resistance of liver cancer to radiotherapy through the IL-4-activated ERK pathway; Authors - Yong Liu
human,rat	WB,IP	Acta Pharmacol Sin; JMJD6 protects against isoproterenol-induced cardiac hypertrophy via inhibition of NF-κB activation by demethylating R149 of the p65 subunit; Authors - Zhen Guo
human	ChIP	J Transl Med; JMJD6-BRD4 complex stimulates lncRNA HOTAIR transcription by binding to the promoter region of HOTAIR and induces radioresistance in liver cancer stem cells; Authors - Ruifeng Pei