Involucrin Polyklonaler Antikörper

Involucrin Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 28462-1-AP

Synonyme

involucrin, IVL

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Galerie der Validierungsdaten

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  WB analysis of A431 using 28462-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 28462-1-AP (Involucrin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28462-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28462-1-AP (Involucrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 28462-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 28462-1-AP (Involucrin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 28462-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Involucrin antibody (28462-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A431 using 28462-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Involucrin (28462-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HT-1080.Zellen, HT-29-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Ösophaguskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

28462-1-AP bindet in WB, IHC, IF, FC, ELISA Involucrin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Involucrin fusion protein Ag29585
• Vollständiger Name: involucrin
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: NM_005547
• Gene symbol: IVL
• Gene ID (NCBI): 3713
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Involucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, but it also forms 120 kDa dimers, while different reports provided variable results ranging from 90-140 kDa. (PMID: 11099111, 1503502, 12150517)

Publikationen

Species	Application	Title
human	IF	J Nanobiotechnology; Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration.; Authors - Yunyao Bo
mouse	IF	Int Immunopharmacol; Nicotinamide mononucleotide ameliorates DNFB-induced atopic dermatitis-like symptoms in mice by blocking activation of ROS-mediated JAK2/STAT5 signaling pathway.; Authors - Jie-Fang Gao
mouse	WB,IHC	J Ethnopharmacol; Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes.; Authors - Zhenzhuo Li
human	WB	J Oncol; Downregulation of S100A9 Reverses Cisplatin-Resistance and Inhibits Proliferation and Migration in Hypopharyngeal Carcinoma; Authors - Shiyu Zeng
human	WB	Biol Pharm Bull; Pyridoxine Has a Potential to Prevent the Appearance of Pigmented Spots: Effects on the Phagocytosis and Differentiation of Keratinocytes; Authors - Yushi Katsuyama
human,mouse	WB	Front Pharmacol; Protective effect of Saussurea involucrata polysaccharide against skin dryness induced by ultraviolet radiation; Authors - Lusheng Wang