CD61 / Integrin Beta 3 Monoklonaler Antikörper

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• KD/KO Validated

CD61 / Integrin Beta 3 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

3D8E5

Kat-Nr. : 66952-1-Ig

Synonyme

CD61, GP3A, GPIIIa, Integrin beta 3, ITGB3

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Galerie der Validierungsdaten

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  WB analysis of HUVEC using 66952-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66952-1-Ig (CD61 / Integrin Beta 3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 66952-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66952-1-Ig (CD61 / Integrin Beta 3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 66952-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66952-1-Ig (CD61 / Integrin Beta 3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66952-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66952-1-Ig (CD61 / Integrin Beta 3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Produktinformation

66952-1-Ig bindet in WB, IP, IHC, ELISA CD61 / Integrin Beta 3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD61 / Integrin Beta 3 fusion protein Ag27834
• Vollständiger Name: integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• Berechnetes Molekulargewicht: 788 aa, 87 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC127666
• Gene symbol: ITGB3
• Gene ID (NCBI): 3690
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Sci Rep; Periostin secreted by activated fibroblasts in idiopathic pulmonary fibrosis promotes tumorigenesis of non-small cell lung cancer.; Authors - Hiroyuki Yamato ; KD Validated
human	WB	Reprod Biol Endocrinol; Expression of Talin-1 in endometriosis and its possible role in pathogenesis.; Authors - Xian Tang
human	WB,IP	BMC Biotechnol; αvβ3-targeted sEVs for efficient intracellular delivery of proteins using MFG-E8.; Authors - Junxin Mai
human	WB	Acta Pharmacol Sin; Adiponectin receptor agonist AdipoRon modulates human and mouse platelet function.; Authors - Xiang-Hui Zhou
mouse	WB	Drug Deliv; Suppression for lung metastasis by depletion of collagen I and lysyl oxidase via losartan assisted with paclitaxel-loaded pH-sensitive liposomes in breast cancer; Authors - Li Zhang
human	WB,IP	Biomedicines; ITCH-Mediated Ubiquitylation of ITGB3 Promotes Cell Proliferation and Invasion of Ectopic Endometrial Stromal Cells in Ovarian Endometriosis; Authors - Liansuo Zhang