Integrin Alpha 6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Integrin Alpha 6 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Rezensionen (1)

Kat-Nr. : 27189-1-AP

Synonyme

CD49f, Integrin alpha 6, Integrin alpha-6, integrin, alpha 6, ITGA6, ITGA6B, VLA 6

---

Galerie der Validierungsdaten

• 

  WB analysis of A549 using 27189-1-AP

  WB result of Integrin Alpha 6 antibody (27189-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 6 transfected A549 cells.

• 

  WB analysis using 27189-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27189-1-AP

  WB result of Integrin Alpha 6 antibody (27189-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 6 transfected HepG2 cells.

• 

  WB analysis of A431 using 27189-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 27189-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin Alpha 6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 27189-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin Alpha 6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skin using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27189-1-AP (Integrin Alpha 6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27189-1-AP (Integrin Alpha 6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:200 (under 40x lens).

• 

  FC experiment of A431 using 27189-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Integrin Alpha 6 (27189-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, A431-Zellen, HepG2-Zellen, Maushautgewebe, Rattenlungengewebe
• Erfolgreiche Detektion in IHC: Maushautgewebe, humanes Kolongewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 6 publications below
• IF: See 6 publications below

Produktinformation

27189-1-AP bindet in WB, IHC, IF, FC, ELISA Integrin Alpha 6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, hamster, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Integrin Alpha 6 fusion protein Ag25835
• Vollständiger Name: integrin, alpha 6
• Berechnetes Molekulargewicht: 1073 aa, 119 kDa
• Beobachtetes Molekulargewicht: 110-130 kDa
• GenBank-Zugangsnummer: BC136456
• Gene symbol: ITGA6
• Gene ID (NCBI): 3655
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	ACS Appl Mater Interfaces; A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells; Authors - Xinyang Zhao
bovine	IF	Cells; Comparative Analysis of mRNA and miRNA Expression between Dermal Papilla Cells and Hair Matrix Cells of Hair Follicles in Yak; Authors - Xiaolan Zhang
monkey	WB	Virulence; Role of intestinal extracellular matrix-related signaling in porcine epidemic diarrhea virus infection.; Authors - Yuchen Li
human	WB	Stem Cell Res Ther; Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.; Authors - Ling Leng
Hamster	IF	J Virol; Single-cell analysis of the impact of host-cell heterogeneity on infection with foot and mouth disease virus.; Authors - Xiu Xin
rat	IF	Vascul Pharmacol; Midkine mediates dysfunction of liver sinusoidal endothelial cells through integrin α4 and α6; Authors - Li Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Insa (Verified Customer) (03-22-2023)

Did work very well in ICC

• Applications: Other
• Primary Antibody Dilution: 1:100-1:400
• Cell Tissue Type: iPSC derived Astrocytes

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C