Integrin Alpha 4 Monoklonaler Antikörper

Integrin Alpha 4 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1B6A3

Kat-Nr. : 67040-1-Ig

Synonyme

CD49D, IA4, Integrin alpha 4, Integrin alpha IV, ITGA4, VLA 4 subunit alpha

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Galerie der Validierungsdaten

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  WB analysis using 67040-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67040-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NK-92 using 67040-1-Ig

  NK-92 cells were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 67040-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MOLT-4-Zellen, Jurkat-Zellen, Raji-Zellen, THP-1-Zellen, U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67040-1-Ig bindet in WB, ELISA Integrin Alpha 4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Integrin Alpha 4 fusion protein Ag28024
• Vollständiger Name: integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• Berechnetes Molekulargewicht: 1032 aa, 115 kDa
• Beobachtetes Molekulargewicht: 130-150 kDa
• GenBank-Zugangsnummer: BC156712
• Gene symbol: ITGA4
• Gene ID (NCBI): 3676
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ITGA4, also named as CD49D, belongs to the integrin alpha chain family. ITGA4 is a receptor for fibronectin, VCAM1 and MADCAM1. ITGA4 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. It recognizes the sequence Q-I-D-S in VCAM1. I It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells ITGA4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.

Publikationen

Species	Application	Title
human	WB	Blood Adv; SORT1/LAMP2-mediated Extracellular Vesicle Secretion and Cell Adhesion Are Linked to Lenalidomide Resistance in Multiple Myeloma.; Authors - Tomofumi Yamamoto