IL-2 Polyklonaler Antikörper
IL-2 Polyklonal Antikörper für IHC, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (1)
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 26156-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | PMA, Ionomycin and Brefeldin A treated EL-4 cells |
| Erfolgreiche Detektion in IHC | humanes Leberkarzinomgewebe, humanes Tonsillitisgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Produktinformation
26156-1-AP bindet in WB, IHC, IF, ELISA IL-2 und zeigt Reaktivität mit human, Maus
| Getestete Reaktivität | human, Maus |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | IL-2 fusion protein Ag24079 |
| Vollständiger Name | interleukin 2 |
| Berechnetes Molekulargewicht | 19 kDa |
| GenBank-Zugangsnummer | NM-008366 |
| Gene symbol | Il2 |
| Gene ID (NCBI) | 16183 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
What is the calculated molecular weight of IL2?
19 kDa
Where is IL-2 produced?
IL-2 is primarily produced and secreted by CD4+ helper T cells in secondary lymphoid organs. CD8+ T cells, some B cells, and dendritic cells also produce IL-2.
What allows the protein to bind to its receptor?
The N-terminal 20 amino acids are essential for protein-receptor interaction.
What are the post-translational modifications of IL-2?
IL-2 may present glycosylation, yet the function of this modification has not been confirmed.
What is the role of IL-2?
IL-2 is a key immunoregulatory cytokine that promotes proliferation of helper T cells, upregulating its own secretion, while also increasing activity of regulatory T cells and providing negative feedback to its production and the immune response as a whole. IL-2 is also a growth factor for natural killer cells and an upregulator of B cell antibody expression and secretion.
What activates the IL-2 pathway?
Combined T cell antigen receptor and CD28 signals allow for maximum activation of the IL-2 pathway.
How does IL-2 expression change during an immune response?
During an immune response, CD4+ and CD8+ T cells will secrete increased amounts of IL-2 to be consumed by regulatory T cells.
How do the responses to strong and weak IL-2 signals differ?
Strong IL-2 levels promote antigen-specific T cells to differentiate into effector T cells, responding immediately to a stimulus or threat. Weak IL-2 signals, however, result in effector T cells to differentiate into helper or memory T cells.
What negatively regulates IL-2?
Immunoregulatory CD4+CD5+ T cells in inhibit IL-2 production by helper T cells.
(PMIDs: 22343569, 24376444, 15473953)
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for IL-2 antibody 26156-1-AP | Protokoll herunterladen |
| IHC protocol for IL-2 antibody 26156-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Magnify is a universal molecular anchoring strategy for expansion microscopy | ||
Invest New Drugs A novel tetravalent bispecific antibody targeting programmed death 1 and tyrosine-protein kinase Met for treatment of gastric cancer. | ||
J Cancer IL-2 enhanced MHC class I expression in papillary thyroid cancer with Hashimoto's thyroiditis overcomes immune escape in vitro. | ||
Peptides Synthesis of potent antagonists of receptors for growth hormone-releasing hormone with antitumor and anti-inflammatory activity. | ||
Lipids Health Dis Arachidonic acid induces macrophage cell cycle arrest through the JNK signaling pathway. | ||
Lung Anti-inflammatory Properties of the Alpha-Melanocyte-Stimulating Hormone in Models of Granulomatous Inflammation. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Lindsey (Verified Customer) (05-24-2021) | Great antibody--worked well from day 1!
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