IL-1 beta Polyklonaler Antikörper

IL-1 beta Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus und mehr (2)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

76

Rezensionen (1)

Kat-Nr. : 26048-1-AP

Synonyme

Il 1b, IL 1beta, IL-1 beta, Il1b, IL-1B, interleukin 1 beta

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Galerie der Validierungsdaten

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  WB analysis using 26048-1-AP

  LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26048-1-AP (IL-1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26048-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26048-1-AP (IL-1 beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  FC experiment of RAW 264.7 using 26048-1-AP

  1X10^6 RAW 264.7 cells untreated (dashed lines) and LPS and Brefeldin A treated (red) were intracellularly stained with 0.4 ug Anti-Mouse IL-1 beta (26048-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.4 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of RAW 264.7 using 26048-1-AP

  1X10^6 RAW 264.7 cells untreated and LPS and Brefeldin A treated were intracellularly stained with 0.4 ug Anti-Mouse IL-1 beta (26048-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen
• Erfolgreiche Detektion in FC: mit LPS und Brefeldin A behandelte RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 60 publications below
• IHC: See 11 publications below
• IF: See 6 publications below
• ELISA: See 1 publications below

Produktinformation

26048-1-AP bindet in WB, IHC, IF, FC, ELISA IL-1 beta und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-1 beta fusion protein Ag23461
• Vollständiger Name: interleukin 1 beta
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: NM_008361
• Gene symbol: Il1b
• Gene ID (NCBI): 16176
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.

Publikationen

Species	Application	Title
human	WB	Nature; Recognition and maturation of IL-18 by caspase-4 noncanonical inflammasome; Authors - Xuyan Shi
rat	IHC	Signal Transduct Target Ther; Metabolic reprogramming of proinflammatory macrophages by target delivered roburic acid effectively ameliorates rheumatoid arthritis symptoms; Authors - Na Jia
human,rat	WB	ACS Nano; Hepatic Stellate Cell- and Liver Microbiome-Specific Delivery System for Dihydrotanshinone I to Ameliorate Liver Fibrosis; Authors - Xia Niu
mouse	WB	Acta Pharm Sin B; Ligand-based substituent-anchoring design of selective receptor-interacting protein kinase 1 necroptosis inhibitors for ulcerative colitis therapy; Authors - Jing Zhu
rat	WB,IF	J Control Release; Controlled extracellular vesicles release from aminoguanidine nanoparticle-loaded polylysine hydrogel for synergistic treatment of spinal cord injury; Authors - Shaoke Wang
mouse	IF	Acta Biomater; ROS-responsive magnesium-containing microspheres for antioxidative treatment of intervertebral disc degeneration; Authors - Tianhui Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marina (Verified Customer) (06-29-2021)

I detected pro-il1b from lysate of macrophages using a dilution of 1:1000, and exposing the membrane for 30sec. Really good and specific antibody. I strongly recommend

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Macrophages

IL-1 beta Antibody Western Blot validation (1:1000 dilution) in Macrophages (Cat no:26048-1-AP)