IL-17a Polyklonaler Antikörper

IL-17a Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 26163-1-AP

Synonyme

Ctla 8, Ctla8, Il17, Il17a, Il-17a, interleukin 17A

---

Galerie der Validierungsdaten

• 

  WB analysis using 26163-1-AP

  PMA and ionomycin treated EL-4 cells were subjected to SDS PAGE followed by western blot with 26163-1-AP (IL-17a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse spleen using 26163-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26163-1-AP (Il17a Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 26163-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26163-1-AP (Il17a Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 26163-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26163-1-AP (IL-17a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 26163-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26163-1-AP (IL-17a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 26163-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26163-1-AP (IL-17a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 26163-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26163-1-AP (IL-17a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 26163-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using 26163-1-AP (Il17a antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 26163-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using 26163-1-AP (Il17a antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PMA, Ionomycin and Brefeldin A treated EL-4 cells
• Erfolgreiche Detektion in IHC: Mausmilzgewebe, humanes Leberkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 8 publications below
• IF: See 5 publications below

Produktinformation

26163-1-AP bindet in WB, IHC, IF, ELISA IL-17a und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-17a fusion protein Ag24087
• Vollständiger Name: interleukin 17A
• GenBank-Zugangsnummer: NM-010552
• Gene symbol: Il17a
• Gene ID (NCBI): 16171
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The IL17A monomer is a peptide consisting of 155 amino acids. The IL17A peptide comprises a 23 amino acid signal peptide and a 132 amino acid mature peptide. The IL17A homodimer has a molecular weight of 35 kD (Kolls and Lindén, 2004).

Publikationen

Species	Application	Title
mouse	IHC	Br J Pharmacol; Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A; Authors - Lu Zhang
human	WB	Cell Biol Toxicol; Interleukin-17A mediates tobacco smoke-induced lung cancer epithelial-mesenchymal transition through transcriptional regulation of ΔNp63α on miR-19.; Authors - Chunfeng Xie
human,mouse	IHC	J Cell Mol Med; Ozone therapy promotes the differentiation of basal keratinocytes via increasing Tp63-mediated transcription of KRT10 to improve psoriasis.; Authors - Lihua Gao
mouse	IHC	Biochim Biophys Acta Mol Cell Res; NEK2 overexpression aggravates IL-22-induced keratinocyte proliferation and cytokine level increases and IMQ-induced psoriasis-like dermatitis; Authors - Youhua Peng
mouse	WB	Front Immunol; Critical Role of AdipoR1 in Regulating Th17 Cell Differentiation Through Modulation of HIF-1α-Dependent Glycolysis.; Authors - Qian Zhang
mouse	WB	Front Immunol; IL-17 Aggravates Pseudomonas aeruginosa Airway Infection in Acute Exacerbations of Chronic Obstructive Pulmonary Disease.; Authors - Fengming Ding