IgG2a Isotype Control Monoklonaler Antikörper

IgG2a Isotype Control Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A3

Kat-Nr. : CL488-65209

Synonyme

IgG2a, IgG2a Isotype Control, Rat IgG2a Isotype Control

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Galerie der Validierungsdaten

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  FC experiment of C57BL/6 mouse splenocytes using CL488-65209

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Rat IgG2a Isotype Control (CL488-65209, Clone:2A3) (red), or 0.5 ug CoraLite® Plus 488 Anti-Mouse CD16/32 (93) (CL488-65057, Clone: 93) (green) or unstained (blue). Cells were not fixed.

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Geprüfte Anwendungen

Erfolgreiche Detektion in FC

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65209 bindet in FC IgG2a Isotype Control und zeigt Reaktivität mit

• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: IgG2a Isotype Control
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

The 2A3 immunoglobulin is useful as an isotype-matched control. The 2A3 immunolglobulin binds to TNP (trinitrophenol), a hapten not expressed on mouse or human cells. It is used as an isotype control for rat IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.