IgG2a Isotype Control Monoklonaler Antikörper

IgG2a Isotype Control Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

Anwendung

IP, FC

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

C1.18.4

Kat-Nr. : 65208-1-Ig

Synonyme

IgG2a, IgG2a Isotype Control, Mouse IgG2a Isotype Control

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Galerie der Validierungsdaten

• 

  FC experiment of human PBMCs using 65208-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Mouse IgG2a Isotype Control (65208-1-Ig, Clone:C1.18.4) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or Anti-Human CD3 (Hit3a) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IP: See 1 publications below

Produktinformation

65208-1-Ig bindet in IP, FC IgG2a Isotype Control und zeigt Reaktivität mit

• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: IgG2a Isotype Control
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

The C1.18.4 immunoglobulin is useful as an isotype-matched control. The C.1.18.4 immunolglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.

Publikationen

Species	Application	Title
	IP	Nucleic Acids Res; Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification; Authors - Hirotatsu Imai