IWS1 Polyklonaler Antikörper

IWS1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 16943-1-AP

Synonyme

IWS1, IWS1 homolog (S. cerevisiae), IWS1 like protein, IWS1L, Protein IWS1 homolog

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Galerie der Validierungsdaten

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  WB analysis using 16943-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16943-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16943-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 16943-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16943-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16943-1-AP

  IP Result of anti-IWS1 (IP:16943-1-AP, 4ug; Detection:16943-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Hirngewebe, Jurkat-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• ChIP: See 1 publications below

Produktinformation

16943-1-AP bindet in WB, IP, ChIP, ELISA IWS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IWS1 fusion protein Ag10554
• Vollständiger Name: IWS1 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 819 aa, 92 kDa
• Beobachtetes Molekulargewicht: 116-120 kDa
• GenBank-Zugangsnummer: BC017012
• Gene symbol: IWS1
• Gene ID (NCBI): 55677
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	ChIP	Cell Host Microbe; The Integrase Cofactor LEDGF/p75 Associates with Iws1 and Spt6 for Postintegration Silencing of HIV-1 Gene Expression in Latently Infected Cells.; Authors - Annabelle Gérard

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sarah (Verified Customer) (12-31-2020)

Works perfectly in WB and immunoprecipitation experiements (HeLa and HEL293T cells).

• Applications: Western Blot, Immunoprecipitation, ChIP
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa and HEK293T