Involucrin Polyklonaler Antikörper

Involucrin Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55328-1-AP

Synonyme

involucrin, IVL



Geprüfte Anwendungen

Erfolgreiche Detektion in WBRaji-Zellen, NIH/3T3-Zellen
Erfolgreiche Detektion in IHChumanes Hautkrebsgewebe, humanes Ösophagusgewebe, humanes Thymusgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFMaushautgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

55328-1-AP bindet in WB, IHC, IF, ELISA Involucrin und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name involucrin
Berechnetes Molekulargewicht 69 kDa
Beobachtetes Molekulargewicht 80-90 kDa, 56-70 kDa
GenBank-ZugangsnummerNM_005547
Gene symbol IVL
Gene ID (NCBI) 3713
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20℃ lagern. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Involucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, while different reports provided variable results ranging from 90-140 kDa. (PMID: 11099111, 1503502)

Protokolle

Produktspezifische Protokolle
WB protocol for Involucrin antibody 55328-1-APProtokoll herunterladen
IHC protocol for Involucrin antibody 55328-1-APProtokoll herunterladen
IF protocol for Involucrin antibody 55328-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

Sci Rep

Loss-of-function Mutation in PMVK Causes Autosomal Dominant Disseminated Superficial Porokeratosis.

Authors - Jiuxiang Wang
humanWB

J Eur Acad Dermatol Venereol

Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.

Authors - T Yuan
humanWB

Oncotarget

Transcriptional suppression of microRNA-27a contributes to laryngeal cancer differentiation via GSK-3β-involved Wnt/β-catenin pathway.

Authors - Sheng Chen
humanWB

Front Oncol

Cystatin-B Negatively Regulates the Malignant Characteristics of Oral Squamous Cell Carcinoma Possibly Via the Epithelium Proliferation/Differentiation Program

Authors - Tian-Tian Xu
humanWB

Int J Mol Sci

Water-Soluble Fullerenol C60(OH)36 toward Effective Anti-Air Pollution Induced by Urban Particulate Matter in HaCaT Cell.

Authors - Chiang-Wen Lee
mouseIHC

Mar Drugs

Fucoidan from Undaria pinnatifida Ameliorates Epidermal Barrier Disruption via Keratinocyte Differentiation and CaSR Level Regulation.

Authors - Yu Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joshua (Verified Customer) (07-27-2019)

Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Moderate staining with minimal background.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human Keratinocytes
Involucrin Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:55328-1-AP)