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ITPR1-specific Polyklonaler Antikörper

ITPR1-specific Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-19962

Synonyme

INSP3R1, IP3 receptor isoform 1, IP3R, IP3R 1, IP3R1, ITPR1, SCA15, SCA16, Type 1 InsP3 receptor

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated ITPR1-specific Polyc (CL488-19962)

    IF Staining of mouse brain using CL488-19962

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 ITPR1-specific antibody (CL488-19962) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated ITPR1-specific Polyc (CL488-19962)

    FC experiment of HepG2 using CL488-19962

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ITPR1-specific (CL488-19962) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFMaushirngewebe
Erfolgreiche Detektion in FCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-19962 bindet in IF, FC (Intra) ITPR1-specific und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name inositol 1,4,5-triphosphate receptor, type 1
Berechnetes Molekulargewicht 314 kDa
Beobachtetes Molekulargewicht 290-300 kDa
GenBank-ZugangsnummerNM_001099952
Gene symbol ITPR1
Gene ID (NCBI) 3708
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ITPR1, also named as INSP3R1 and IP3R, belongs to the InsP3 receptor family. It is a receptor for inositol 1,4,5-trisphosphate which is a second messenger that mediates the release of intracellular calcium. Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15). The antibody has no cross reaction with ITPR2 and ITPR3.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 ITPR1-specific antibody CL488-19962Protokoll herunterladen
FC protocol for CL Plus 488 ITPR1-specific antibody CL488-19962Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen