ITPR1-specific Polyklonaler Antikörper

ITPR1-specific Polyklonal Antikörper für FC, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Kat-Nr. : 19962-1-AP

Synonyme

INSP3R1, IP3 receptor isoform 1, IP3R, IP3R 1, IP3R1, ITPR1, SCA15, SCA16, Type 1 InsP3 receptor

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 19962-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19962-1-AP (ITPR1-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19962-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19962-1-AP (ITPR1-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 19962-1-AP

  IP result of anti-ITPR1-specific (IP:19962-1-AP, 4ug; Detection:19962-1-AP 1:300) with mouse brain tissue lysate 3160 ug.

• 

  IHC staining of human brain using 19962-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 19962-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 19962-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 19962-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse brain using 19962-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19962-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 19962-1-AP

  1X10^6 HepG2 cells were stained with 0.2 ug ITPR1-specific antibody (19962-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mauslebergewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Hodengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 19 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Produktinformation

19962-1-AP bindet in WB, IP, IHC, FC, ELISA ITPR1-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ente, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: inositol 1,4,5-triphosphate receptor, type 1
• Berechnetes Molekulargewicht: 314 kDa
• Beobachtetes Molekulargewicht: 290-300 kDa
• GenBank-Zugangsnummer: NM_001099952
• Gene symbol: ITPR1
• Gene ID (NCBI): 3708
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ITPR1, also named as INSP3R1 and IP3R, belongs to the InsP3 receptor family. It is a receptor for inositol 1,4,5-trisphosphate which is a second messenger that mediates the release of intracellular calcium. Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15). The antibody has no cross reaction with ITPR2 and ITPR3.

Publikationen

Species	Application	Title
human		Neuron; Nicotinamide Pathway-Dependent Sirt1 Activation Restores Calcium Homeostasis to Achieve Neuroprotection in Spinocerebellar Ataxia Type 7.; Authors - Colleen A Stoyas
mouse	WB	Mol Psychiatry; A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.; Authors - Yue Qin
mouse		Diabetes; PACS-2 Ameliorates Tubular Injury by Facilitating Endoplasmic Reticulum-Mitochondria Contact and Mitophagy in Diabetic Nephropathy.; Authors - Chenrui Li
human	WB	Cancer Lett; MORTALIN-Ca2+ axis drives innate rituximab resistance in diffuse large B-cell lymphoma.; Authors - Qi Sun
rat	WB	Mol Nutr Food Res; Sulforaphane Balances Ca2+ Homeostasis Injured by Excessive Fat via Mitochondria-Associated Membrane (MAM).; Authors - Sicong Tian
rat	WB	Front Immunol; Interleukin-6-Mediated-Ca2+ Handling Abnormalities Contributes to Atrial Fibrillation in Sterile Pericarditis Rats.; Authors - Jie Liao