ITPKA Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ITPKA Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 14270-1-AP

Synonyme

InsP 3 kinase A, IP3 3 kinase A, IP3K A, IP3KA, ITPKA

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 14270-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14270-1-AP (ITPKA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14270-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14270-1-AP (ITPKA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14270-1-AP

  IP Result of anti-ITPKA (IP:14270-1-AP, 4ug; Detection:14270-1-AP 1:400) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 14270-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14270-1-AP (ITPKA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14270-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14270-1-AP (ITPKA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14270-1-AP

  Immunofluorescent analysis of HepG2 cells, using ITPKA antibody 14270-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3200
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

14270-1-AP bindet in WB, IP, IHC, IF, ELISA ITPKA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ITPKA fusion protein Ag5588
• Vollständiger Name: inositol 1,4,5-trisphosphate 3-kinase A
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC026331
• Gene symbol: ITPKA
• Gene ID (NCBI): 3706
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ITPKA(Inositol-trisphosphate 3-kinase A) belongs to the inositol phosphokinase (IPK) family. It accumulates in dendritic spines and seems to be critically involved in synaptic plasticity. The protein possesses two functional activities. In neurons, ITPKA is targeted to F-actin via an N-terminal actin binding domain (amino acids 1-66) and is relevant for long term potentiation and spatial learning(PMID:20022963).

Publikationen

Species	Application	Title
human		Neuron; Nicotinamide Pathway-Dependent Sirt1 Activation Restores Calcium Homeostasis to Achieve Neuroprotection in Spinocerebellar Ataxia Type 7.; Authors - Colleen A Stoyas
mouse	IHC	J Mol Cell Biol; Single-cell transcriptomes reveal molecular specializations of neuronal cell types in the developing cerebellum.; Authors - Jian Peng
human	WB,IHC	Int J Cancer; Functional role of inositol-1,4,5-trisphosphate-3-kinase-A for motility of malignant transformed cells.; Authors - Windhorst Sabine S ; KD Validated
mouse	WB	Neuroscience; Sex-specific Regulation of Spine Density and Synaptic Proteins by G-protein-coupled Estrogen Receptor (GPER)1 in Developing Hippocampus.; Authors - Xiaoyu Li
human	WB, IF	Cytoskeleton (Hoboken); The catalytic domain of inositol-1,4,5-trisphosphate 3-kinase-A (ITPKA) contributes to ITPKA-induced modulation of F-actin.; Authors - Dina Ashour
human	WB	Diagn Pathol; ITPKA expression is a novel prognostic factor in hepatocellular carcinoma.; Authors - Jianbiao Li