ITM2A Polyklonaler Antikörper

ITM2A Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 18306-1-AP

Synonyme

BRICD2A, E25A, integral membrane protein 2A, ITM2A, Protein E25

---

Galerie der Validierungsdaten

• 

  WB analysis using 18306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18306-1-AP (ITM2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18306-1-AP (ITM2A antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of Jurkat using 18306-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human ITM2A (18306-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• FC: See 1 publications below

Produktinformation

18306-1-AP bindet in WB, IHC, IF, FC, ELISA ITM2A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: integral membrane protein 2A
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC040437
• Gene symbol: ITM2A
• Gene ID (NCBI): 9452
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ITM2A, also named BRICD2A and E25A, is a member of type II integral membrane (ITM2) protein family. ITM2A expression is high in osteogenic and lymphoid tissues, while both ITM2B and ITM2C are expressed in brain. There are 5 articles specifically referring to this gene in PubMed. Proteins are expected to localize in various compartments (cytoplasm, integral to membrane). No phenotype has yet been reported to our knowledge: this gene's in vivo function is yet unknown.

Publikationen

Species	Application	Title
mouse	IHC	Bone Res; Single cell analysis reveals inhibition of angiogenesis attenuates the progression of heterotopic ossification in Mkx-/- mice.; Authors - Junxin Lin
human	FC	Blood; Identification of early gene expression changes during human Th17 cell differentiation.; Authors - Tuomela Soile S
human	WB,IHC,IF	Cell Commun Signal; Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction.; Authors - Cefan Zhou
human,mouse	IF	Fluids Barriers CNS; Exploring ITM2A as a new potential target for brain delivery.; Authors - Céline Cegarra
mouse	IF	iScience; The regenerating skeletal muscle niche drives satellite cell return to quiescence.; Authors - Alicia A Cutler
mouse	IHC	PLoS One; Itm2a expression in the developing mouse first lower molar, and the subcellular localization of itm2a in mouse dental epithelial cells.; Authors - Makiko Kihara

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C