ITIH3 Polyklonaler Antikörper

ITIH3 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 21247-1-AP

Synonyme

Inter-alpha-inhibitor heavy chain 3, Inter-alpha-trypsin inhibitor heavy chain H3, ITI HC3, ITI heavy chain H3, ITI-HC3

---

• 

  WB analysis of human plasma using 21247-1-AP

  human serum were subjected to SDS PAGE followed by western blot with 21247-1-AP (ITIH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human plasma using 21247-1-AP

  IP result of anti-ITIH3 (IP:21247-1-AP, 4ug; Detection:21247-1-AP 1:1000) with human plasma lysate 4000ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Serumgewebe
• Erfolgreiche IP: humanes Plasmagewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

21247-1-AP bindet in WB, IHC, IP, ELISA ITIH3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ITIH3 fusion protein Ag15750
• Vollständiger Name: inter-alpha (globulin) inhibitor H3
• Berechnetes Molekulargewicht: 890 aa, 100 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC107604
• Gene symbol: ITIH3
• Gene ID (NCBI): 3699
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ITIH3, or inter-alpha-trypsin inhibitor heavy chain 3, is a gene in humans that encodes the heavy chain subunit of the pre-alpha-trypsin inhibitor complex. This complex plays a role in stabilizing the extracellular matrix through its ability to bind hyaluronic acid. The gene is located on chromosome 3 and is part of the inter-alpha-trypsin inhibitor family gene cluster. ITIH3 has restricted expression primarily toward the liver, with an RPKM value of 228.8, indicating a relatively high level of expression in this organ.

Publikationen

Species	Application	Title
human	IHC	Br J Cancer; A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.; Authors - Xiaohui Liu
human	WB	J Pers Med; Comprehensive Analysis and Experimental Validation of a Novel Estrogen/Progesterone-Related Prognostic Signature for Endometrial Cancer.; Authors - Jing Yu
	WB	Sci Rep; Plasma Protein Comparison between Dairy Cows with Inactive Ovaries and Estrus.; Authors - Chang Zhao
human	IHC	Dis Markers; Plasma Inter-Alpha-Trypsin Inhibitor Heavy Chains H3 and H4 Serve as Novel Diagnostic Biomarkers in Human Colorectal Cancer.; Authors - Xiao Jiang