Integrin Alpha V Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Integrin Alpha V Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 27096-1-AP

Synonyme

CD51, Integrin alpha V, ITGAV, MSK8, VNRA

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Galerie der Validierungsdaten

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  WB analysis using 27096-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27096-1-AP (Integrin Alpha V antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27096-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27096-1-AP (Integrin Alpha V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27096-1-AP (Integrin Alpha V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 27096-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Integrin Alpha V antibody (27096-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, A549-Zellen, humanes Plazenta-Gewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Leberkarzinomgewebe, humanes Magenkrebsgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below

Produktinformation

27096-1-AP bindet in WB, IHC, IF, ELISA Integrin Alpha V und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Integrin Alpha V fusion protein Ag25825
• Vollständiger Name: integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
• Berechnetes Molekulargewicht: 1048 aa, 116 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC136442
• Gene symbol: ITGAV
• Gene ID (NCBI): 3685
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Aging Cell; Fibronectin type III domain-containing 5 improves aging-related cardiac dysfunction in mice.; Authors - Can Hu ; KD Validated
human	WB	Curr Biol; The viral restriction factor tetherin/BST2 tethers cytokinetic midbody remnants to the cell surface.; Authors - Adrien Presle
human	WB,IHC	Int J Mol Sci; Macrophages Cytokine Spp1 Increases Growth of Prostate Intraepithelial Neoplasia to Promote Prostate Tumor Progression.; Authors - Justin K Messex
human	WB	Int J Mol Sci; Identification of Novel microRNA Profiles Dysregulated in Plasma and Tissue of Abdominal Aortic Aneurysm Patients.; Authors - Emma Plana
human	IHC	Cancer Cell Int; Bioinformatics combined with quantitative proteomics analyses and identification of potential biomarkers in cholangiocarcinoma.; Authors - Zijian Da
human	WB	J Exp Clin Cancer Res; Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer.; Authors - Feikai Lin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (04-24-2019)

By WB, this antibody gave a clean band at the expected molecular weight; by IF, it could not label a specific pattern.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF