Integrin Alpha 4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Integrin Alpha 4 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Rezensionen (1)

Kat-Nr. : 19676-1-AP

Synonyme

CD49D, IA4, Integrin alpha 4, Integrin alpha IV, ITGA4, VLA 4 subunit alpha

---

Galerie der Validierungsdaten

• 

  WB analysis using 19676-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19676-1-AP (Integrin alpha-4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 19676-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19676-1-AP (Integrin alpha-4 Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 19676-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19676-1-AP (Integrin alpha-4 Antibody) at dilution of 1:50.

• 

  FC experiment of Jurkat using 19676-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human Integrin Alpha 4 (19676-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

19676-1-AP bindet in WB, IHC, IF, FC, ELISA Integrin Alpha 4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• Berechnetes Molekulargewicht: 115 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: NM_000885
• Gene symbol: ITGA4
• Gene ID (NCBI): 3676
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ITGA4, also named as CD49D, belongs to the integrin alpha chain family. ITGA4 is a receptor for fibronectin, VCAM1 and MADCAM1. ITGA4 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. It recognizes the sequence Q-I-D-S in VCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells ITGA4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Calreticulin and integrin alpha dissociation induces anti-inflammatory programming in animal models of inflammatory bowel disease.; Authors - Masayoshi Ohkuro
mouse	WB	J Exp Med; EP3 enhances adhesion and cytotoxicity of NK cells toward hepatic stellate cells in a murine liver fibrosis model.; Authors - Xixi Tao
human	WB	Int J Biol Macromol; Diethylaminoethyl-dextran and monocyte cell membrane coated 1,8-cineole delivery system for intracellular delivery and synergistic treatment of atherosclerosis; Authors - Feng Jiang
human,mouse	WB	Int J Mol Sci; Induced Pluripotent Stem Cell-Derived Conditioned Medium Promotes Endogenous Leukemia Inhibitory Factor to Attenuate Endotoxin-Induced Acute Lung Injury.; Authors - Vincent Yi-Fong Su
human	WB	Int J Mol Sci; Sesquiterpene Lactone Deoxyelephantopin Isolated from Elephantopus scaber and Its Derivative DETD-35 Suppress BRAFV600E Mutant Melanoma Lung Metastasis in Mice.; Authors - Biljana Cvetanova
human	WB	J Neuroinflammation; Downregulation of CD151 restricts VCAM-1 mediated leukocyte infiltration to reduce neurobiological injuries after experimental stroke.; Authors - Ceshu Gao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Boyan (Verified Customer) (03-11-2019)

For WB, the expected band was very weak, and was much weaker than those non-specific bands; for IF, it could label the cell cortex localisation of Itga4, partially co-localised with Actin.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF