Integrin alpha-1 Polyklonaler Antikörper

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• KD/KO Validated

Integrin alpha-1 Polyklonal Antikörper für FC, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, FC

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (1)

Kat-Nr. : 22146-1-AP

Synonyme

CD49a, Integrin alpha 1, integrin, alpha 1, ITGA1, VLA 1, VLA1

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 22146-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22146-1-AP (ITGA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 22146-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 22146-1-AP (ITGA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22146-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22146-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 22146-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22146-1-AP (Integrin Alpha 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 22146-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22146-1-AP (Integrin Alpha 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 22146-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 22146-1-AP (ITGA1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 22146-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 22146-1-AP (ITGA1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 22146-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Integrin alpha-1 antibody (22146-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

22146-1-AP bindet in WB, IHC, IF, FC Integrin alpha-1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Integrin alpha-1 fusion protein Ag17584
• Vollständiger Name: integrin, alpha 1
• Berechnetes Molekulargewicht: 1179 aa, 131 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: BC137121
• Gene symbol: ITGA1
• Gene ID (NCBI): 3672
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).

Publikationen

Species	Application	Title
human	WB	Int J Biol Sci; Integrin α1 promotes tumorigenicity and progressive capacity of colorectal cancer.; Authors - Hai Li ; KD Validated
human	WB	Int J Oncol; Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules; Authors - AMINA BOLATKAN
human	WB	J Ethnopharmacol; A combination index and glycoproteomics-based approach revealed synergistic anticancer effects of curcuminoids of turmeric against prostate cancer PC3 cells.; Authors - Meng-Meng Wei
human	WB,IHC,IF	Int J Oncol; FAM83A exerts tumor‑suppressive roles in cervical cancer by regulating integrins.; Authors - Junfen Xu
rat	WB,IF	J Biomech; Integrin endocytosis on elastic substrates mediates mechanosensing.; Authors - Jing Li
rat	WB	Med Sci Monit; 17β-Estradiol Protects Rat Annulus Fibrosus Cells Against Apoptosis via α1 Integrin-Mediated Adhesion to Type I Collagen: An In-vitro Study.; Authors - Chun-Ming Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (04-24-2019)

This antibody labelled a band above 250 kD, which is likely the glycosylated form of Itga1. But, it did not label any band around 170 kD as shown in the data sheet.

• Applications: Western Blot,
• Cell Tissue Type: MEF