ISG20L2 Polyklonaler Antikörper

ISG20L2 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 24639-1-AP

Synonyme

HSD 38, ISG20L2

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 24639-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24639-1-AP (ISG20L2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 24639-1-AP

  IP Result of anti-ISG20L2 (IP:24639-1-AP, 4ug; Detection:24639-1-AP 1:1000) with HEK-293 cells lysate 3240ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

24639-1-AP bindet in WB, IP, ELISA ISG20L2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ISG20L2 fusion protein Ag20026
• Vollständiger Name: interferon stimulated exonuclease gene 20kDa-like 2
• Berechnetes Molekulargewicht: 353 aa, 39 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC000575
• Gene symbol: ISG20L2
• Gene ID (NCBI): 81875
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ISG20L2, also named HSD38, is a member of a vertebrate exonucleases family. ISG20L2 is composed of at least two functional domains, one at its N-terminal half allowing intracellular localization and association with the binding partners and the other at its C-terminal half bearing the 3` to 5` exoribonuclease activity. The molecular weight of ISG20L2 is 39 kDa (PMID: 18065403).

Publikationen

Species	Application	Title
human	WB	JCI Insight; ISG20L2 suppresses bortezomib anti-myeloma activity by attenuating bortezomib binding to PSMB5; Authors - Yan Yang
human	WB	Nucleic Acids Res; Molecular mechanism of human ISG20L2 for the ITS1 cleavage in the processing of 18S precursor ribosomal RNA; Authors - Yinliang Ma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sara (Verified Customer) (05-24-2023)

It is the best ISG20L2 I have tried by far compared to other commercial ones. I have used it for WB (1:5000) and for immunoprecipitation. I need to mention that we tried it for IF but it did not work properly, at least for Jurkat T cells!

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:5000