IRS2 Polyklonaler Antikörper

IRS2 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 20702-1-AP

Synonyme

IRS 2, IRS2

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Galerie der Validierungsdaten

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  WB analysis using 20702-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20702-1-AP (IRS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 20702-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20702-1-AP (IRS2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse skeletal muscle using 20702-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20702-1-AP (IRS2 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IF: See 1 publications below

Produktinformation

20702-1-AP bindet in WB, IHC, IF, ELISA IRS2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: IRS2
• Berechnetes Molekulargewicht: 137 kDa
• Beobachtetes Molekulargewicht: 150-185 kDa
• GenBank-Zugangsnummer: NM_003749
• Gene symbol: IRS2
• Gene ID (NCBI): 8660
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The family of INS receptor substrates (IRS) consists of four proteins (IRS-1-IRS-4), which were initially characterized as typical cytosolic adaptor proteins involved in INS receptor (IR) and INS-like growth factor I receptor (IGF-IR) signaling. Impaired glucose tolerance and INS resistance, and thus type 2 diabetes were demonstrated in Irs2 knockout mice. In vivo data also indicated that he regulation of beta-cell mass involves IRS2, while IRS1 is only required for proper INS production in beta-cells. IRS2, like IRS1, has a predicted molecular weight of 137 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS-PAGE as a band of approximately 150-185 kDa.

Publikationen

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; Microbiota from Exercise Mice Counteracts High-Fat High-Cholesterol Diet-Induced Cognitive Impairment in C57BL/6 Mice; Authors - Rui Li
human	WB	Sci Rep; TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis.; Authors - Fengqin Dong
human	WB,IF	Int J Biol Sci; CircFAT1 Promotes Lung Adenocarcinoma Progression by Sequestering miR-7 from Repressing IRS2-ERK-mediated CCND1 Expression.; Authors - Hang Peng
rat	WB	Food Funct; Elucidation of the interaction effect between dietary fiber and bound polyphenol components on the anti-hyperglycemic activity of tea residue dietary fiber.; Authors - Hairong Huang
human,mouse	WB	Heliyon; The effect of PINK1/Parkin pathway on glucose homeostasis imbalance induced by tacrolimus in mouse livers; Authors - Zhiwei Li
mouse	WB	Obesity (Silver Spring); MiR-195 promotes pancreatic β-cell dedifferentiation by targeting Mfn2 and impairing Pi3k/Akt signaling in type 2 diabetes.; Authors - Yuhua Xu