IRF5 Monoklonaler Antikörper

IRF5 Monoklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

2A10C5

Kat-Nr. : 60088-1-Ig

Synonyme

interferon regulatory factor 5, IRF 5, IRF5, SLEB10

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 60088-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60088-1-Ig (IRF5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 60088-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60088-1-Ig (IRF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 60088-1-Ig

  IP Result of anti-IRF5 (IP:60088-1-Ig, 4ug; Detection:60088-1-Ig 1:500) with HeLa cells lysate 1600ug.

• 

  IHC staining of human breast cancer using 60088-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60088-1-Ig (IRF5 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 60088-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60088-1-Ig (IRF5 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 60088-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60088-1-Ig (IRF5 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 60088-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60088-1-Ig (IRF5 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 60088-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60088-1-Ig (IRF5 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 60088-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60088-1-Ig (IRF5 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60088-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60088-1-Ig (IRF5 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60088-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60088-1-Ig (IRF5 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 60088-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60088-1-Ig (IRF5 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

60088-1-Ig bindet in WB, IP, IHC, IF, ELISA IRF5 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IRF5 fusion protein Ag0841
• Vollständiger Name: interferon regulatory factor 5
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 63+110 kDa
• GenBank-Zugangsnummer: BC004201
• Gene symbol: IRF5
• Gene ID (NCBI): 3663
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. I t is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10).

Publikationen

Species	Application	Title
human	WB	J Virol; Manipulation of the toll-like receptor 7 signaling pathway by Epstein-Barr virus.; Authors - Martin Heather J HJ