- Featured Product
- KD/KO Validated
IRF3 Polyklonaler Antikörper
IRF3 Polyklonal Antikörper für FC (Intra), IF
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human
Anwendung
IF, FC (Intra)
Konjugation
CoraLite® Plus 488 Fluorescent Dye
Kat-Nr. : CL488-11312
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in IF | HepG2-Zellen |
Erfolgreiche Detektion in FC | HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Immunfluoreszenz (IF) | IF : 1:50-1:500 |
Durchflusszytometrie (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
CL488-11312 bindet in IF, FC (Intra) IRF3 und zeigt Reaktivität mit human
Getestete Reaktivität | human |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | IRF3 fusion protein Ag1858 |
Vollständiger Name | interferon regulatory factor 3 |
Berechnetes Molekulargewicht | 47 kDa |
Beobachtetes Molekulargewicht | 50-60 kDa |
GenBank-Zugangsnummer | BC009395 |
Gene symbol | IRF3 |
Gene ID (NCBI) | 3661 |
Konjugation | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3. |
Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. IRF3 exists some isoforms with MV 47-49 kDa, 33 kDa and 12-16 kDa.
Protokolle
Produktspezifische Protokolle | |
---|---|
IF protocol for CL Plus 488 IRF3 antibody CL488-11312 | Protokoll herunterladen |
FC protocol for CL Plus 488 IRF3 antibody CL488-11312 | Protokoll herunterladen |
Standard-Protokolle | |
---|---|
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |