IRE1; ERN1 Polyklonaler Antikörper

IRE1; ERN1 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

80

Kat-Nr. : 27528-1-AP

Synonyme

ERN1, hIRE1p, Inositol requiring protein 1, IRE1, Ire1 alpha, IRE1; ERN1, IRE1P, IRE1α

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Galerie der Validierungsdaten

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  WB analysis using 27528-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27528-1-AP (IRE1; ERN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 27528-1-AP

  IP result of anti-IRE1; ERN1(IP:27528-1-AP, 4ug; Detection:27528-1-AP 1:500) with A549 cells lysate 600 ug.

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  IHC staining of human pancreas cancer using 27528-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27528-1-AP (IRE1; ERN1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas cancer using 27528-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27528-1-AP (IRE1; ERN1 antibody) at dilution of 1:200 (under 40x lens).

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  FC experiment of A549 using 27528-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human IRE1; ERN1 (27528-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, LNCaP-Zellen, PC-3-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 74 publications below
• IHC: See 6 publications below
• IF: See 7 publications below

Produktinformation

27528-1-AP bindet in WB, IP, IHC, IF, FC, ELISA IRE1; ERN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IRE1; ERN1 fusion protein Ag25580
• Vollständiger Name: endoplasmic reticulum to nucleus signaling 1
• Berechnetes Molekulargewicht: 977 aa, 110 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC130405
• Gene symbol: ERN1
• Gene ID (NCBI): 2081
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Inositol-requiring enzyme 1 (IRE1) is an ER transmembrane sensor that activates the UPR to maintain the ER and cellular function. Although mammalian IRE1 promotes cell survival, it can initiate apoptosis via decay of antiapoptotic miRNAs. IRE1 contains a ribonuclease domain in its cytoplasmic region which initiates splicing reaction by direct cleavage of XBP1 mRNA at the two stem loop structures. IRE1 plays instrumental protumoral roles in several cancers, and high IRE1 activity has been associated with poorer prognoses. (PMID: 23880584, PMID: 32861679, PMID: 31875595)

Publikationen

Species	Application	Title
human	WB	Cell; Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.; Authors - Rui Yang
	WB	Cell Stem Cell; Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis.; Authors - Changzheng Li
	WB	Redox Biol; FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response; Authors - Zhe Wang
human	WB	Genome Biol; Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes; Authors - Iliana Soto
	WB	J Hazard Mater; Endoplasmic reticulum stress manipulates autophagic response that antagonizes polybrominated diphenyl ethers quinone induced cytotoxicity in microglial BV2 cells.; Authors - Qiushuang Zhu
rat	WB	Redox Biol; Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.; Authors - Yuehong Wang