IRAK1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

IRAK1 Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1H10A7

Kat-Nr. : 66653-1-Ig

Synonyme

IRAK, IRAK 1, IRAK1, pelle

---

Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 66653-1-Ig

  WB result of IRAK1 antibody (66653-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRAK1 transfected HEK-293 cells.

• 

  WB analysis of HeLa using 66653-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66653-1-Ig (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66653-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66653-1-Ig (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66653-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66653-1-Ig (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66653-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66653-1-Ig (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 66653-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66653-1-Ig (IRAK1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66653-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66653-1-Ig (IRAK1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

66653-1-Ig bindet in WB, IHC, IF, ELISA IRAK1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IRAK1 fusion protein Ag0728
• Vollständiger Name: interleukin-1 receptor-associated kinase 1
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC014963
• Gene symbol: IRAK1
• Gene ID (NCBI): 3654
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)

Publikationen

Species	Application	Title
human	IF	Cell Mol Immunol; Virus-inducible IGFALS facilitates innate immune responses by mediating IRAK1 and TRAF6 activation.; Authors - Gang Xu
human	IF	Cell Death Dis; Radiation induces IRAK1 expression to promote radioresistance by suppressing autophagic cell death via decreasing the ubiquitination of PRDX1 in glioma cells; Authors - Jing Li
human	IHC	Biol Reprod; miR-146b-5p downregulates IRAK1 and ADAM19 to suppress trophoblast proliferation, invasion and migration in miscarriage; Authors - Xiaoli Zhang