IPO7 Polyklonaler Antikörper

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• KD/KO Validated

IPO7 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 28289-1-AP

Synonyme

FLJ14581, Imp7, importin 7, IPO7, Ran binding protein 7, RANBP7

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Galerie der Validierungsdaten

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  WB analysis using 28289-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28289-1-AP (IPO7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 28289-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 28289-1-AP (IPO7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 28289-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28289-1-AP (IPO7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28289-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28289-1-AP (IPO7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

28289-1-AP bindet in WB, IHC, IF, ELISA IPO7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IPO7 fusion protein Ag27257
• Vollständiger Name: importin 7
• Berechnetes Molekulargewicht: 120 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: NM_006391.2
• Gene symbol: IPO7
• Gene ID (NCBI): 10527
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Nat Commun; Mechanical control of nuclear import by Importin-7 is regulated by its dominant cargo YAP.; Authors - María García-García
human	WB	J Cell Biol; Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine.; Authors - Chen Chen
mouse	IHC	Cells Dev; The regulation of Msx1 by BMP4/pSmad1/5 signaling is mediated by importin7 in dental mesenchymal cells.; Authors - Yawei She ; KD Validated
human	WB	iScience; Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges; Authors - Tamami Miyagi
human	IHC	Mol Immunol; IPO7 promotes lipopolysaccharide-induced inflammatory responses in human dental pulp cells via p38 MAPK and NF-κB signaling pathways; Authors - Ziqiu Xiao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

María (Verified Customer) (02-08-2022)

Works fine for WB (1:1000) and IF in permeabilized cells (1:100). Validated with siRNA

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RPE-1/MSC