INTS8 Polyklonaler Antikörper

INTS8 Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 18802-1-AP

Synonyme

C8orf52, INT8, integrator complex subunit 8, INTS8, Protein kaonashi 1

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 18802-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18802-1-AP (INTS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18802-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18802-1-AP (INTS8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 18802-1-AP

  IP Result of anti-INTS8 (IP:18802-1-AP, 4ug; Detection:18802-1-AP 1:500) with HeLa cells lysate 4000ug.

• 

  IF Staining of HeLa using 18802-1-AP

  Immunofluorescent analysis of Hela cells, using INTS8 antibody 18802-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

18802-1-AP bindet in WB, IP, IHC, IF, ELISA INTS8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: integrator complex subunit 8
• Berechnetes Molekulargewicht: 111 kDa
• Beobachtetes Molekulargewicht: 90-115 kDa
• GenBank-Zugangsnummer: BC136754
• Gene symbol: INTS8
• Gene ID (NCBI): 55656
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

INTS8, also named as C8orf52, is a Component of the Integrator complex which is a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. INTS8 is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.

Publikationen

Species	Application	Title
mouse	WB	Science; Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.; Authors - Hai Zheng
human	WB	J Exp Clin Cancer Res; Microarray-based identification of genes associated with cancer progression and prognosis in hepatocellular carcinoma.; Authors - Fuqiang Yin
human	IHC	Cancer Manag Res; INTS8 accelerates the epithelial-to-mesenchymal transition in hepatocellular carcinoma by upregulating the TGF-β signaling pathway.; Authors - Hui Tong
human	WB	Cell Rep; A homozygous variant in INTS11 links mitosis and neurogenesis defects to a severe neurodevelopmental disorder; Authors - Hanzhe Kuang