INTS5 Polyklonaler Antikörper

INTS5 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 14069-1-AP

Synonyme

INT5, integrator complex subunit 5, INTS5, KIAA1698

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Galerie der Validierungsdaten

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  WB analysis using 14069-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14069-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14069-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14069-1-AP

  IP Result of anti-INTS5 (IP:14069-1-AP, 4ug; Detection:14069-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of human prostate cancer using 14069-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14069-1-AP (INTS5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14069-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14069-1-AP (INTS5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14069-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14069-1-AP (INTS5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, NIH/3T3-Zellen, SKOV-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

14069-1-AP bindet in WB, IP, IHC, IF, ELISA INTS5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: INTS5 fusion protein Ag5129
• Vollständiger Name: integrator complex subunit 5
• Berechnetes Molekulargewicht: 108 kDa
• Beobachtetes Molekulargewicht: 108 kDa
• GenBank-Zugangsnummer: BC060841
• Gene symbol: INTS5
• Gene ID (NCBI): 80789
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

INTS5, also named as INT5 and KIAA1698, is a component of the Integrator complex which involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.

Publikationen

Species	Application	Title
mouse	WB	Science; Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.; Authors - Hai Zheng
human	WB	Nat Commun; INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex; Authors - Lauren G. Mascibroda
human	WB	Cell Rep; A combinatorial approach to uncover an additional Integrator subunit; Authors - Sarah R Offley
human	WB	Cell Rep; RPAP2 regulates a transcription initiation checkpoint by inhibiting assembly of pre-initiation complex.; Authors - Xinxin Wang
human	WB	Mol Cell; INTAC endonuclease and phosphatase modules differentially regulate transcription by RNA polymerase II; Authors - Shibin Hu