INSR Polyklonaler Antikörper

INSR Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (5)

Anwendung

WB, IP, IHC, Dot blot, CoIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20433-1-AP

Synonyme

CD220, HHF5, INSR, IR



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HeLa-Zellen, HT-1080.Zellen, MCF-7-Zellen
Erfolgreiche IPHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

20433-1-AP bindet in WB, IP, IHC, Dot blot, CoIP, ELISA INSR und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Fisch, Hausschwein, Maus, Ratte, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen INSR fusion protein Ag13922
Vollständiger Name INSR
Berechnetes Molekulargewicht 1382 aa, 156 kDa
Beobachtetes Molekulargewicht 190 kDa, 135 kDa, 95 kDa
GenBank-ZugangsnummerBC117172
Gene symbol INSR
Gene ID (NCBI) 3643
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The biological effects of INS are mediated by a membrane spanning cell surface receptor that has been shown to consist of disulfide-linked 135-kDa and 95-kDa alpha- and beta-subunits that are cleaved products of a common precursor. Binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896). This antibody raised against 980-1382aa of human INS receptor can recognize full-length INS receptor precursor and INS receptor beta subunit.

Protokolle

Produktspezifische Protokolle
WB protocol for INSR antibody 20433-1-APProtokoll herunterladen
IP protocol for INSR antibody 20433-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseDot blot

Environ Health Perspect

Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice.

Authors - Jacob Raber
ratWB

Food Funct

l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways

Authors - Ling Lin
humanWB

Br J Surg

MiR-128 regulation of glucose metabolism and cell proliferation in triple-negative breast cancer.

Authors - M Xiao
humanWB

FASEB J

Angptl7 promotes insulin resistance and type 2 diabetes mellitus by multiple mechanisms including SOCS3-mediated IRS1 degradation.

Authors - Tong Xu
mouseWB,IHC

Sci Rep

NaoXinTong Capsules inhibit the development of diabetic nephropathy in db/db mice.

Authors - Shu Yang
mouseWB

J Mol Med (Berl)

Metabolomics reveals that chronic restraint stress alleviates carbon tetrachloride-induced hepatic fibrosis through the INSR/PI3K/AKT/AMPK pathway

Authors - Shanshan Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Mi (Verified Customer) (05-29-2023)

It works well in human white adipocytes, we get strong signals

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: adipocytes
FH

Chiara (Verified Customer) (10-04-2021)

Works well but three bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: mesenchymal Stem cell
FH

Boyan (Verified Customer) (06-06-2021)

This antibody recognized a band at ~ 180 kd only, which is sensitive to PNGaseF, but not EndoH, treatment. The size is much bigger than an expected InsR band. Importantly, stripping the membrane and re-blot with another InsR antibody from a different brand could detect the protein at the expected MW. These results suggest this InsR antibody is detecting something other than InsR.

  • Applications: Western Blot