INSR Polyklonaler Antikörper
INSR Polyklonal Antikörper für IP, WB,ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (5)
Anwendung
WB, IP, IHC, Dot blot, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 20433-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HEK-293-Zellen, HeLa-Zellen, HT-1080.Zellen, MCF-7-Zellen |
Erfolgreiche IP | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 25 publications below |
IHC | See 1 publications below |
CoIP | See 1 publications below |
Produktinformation
20433-1-AP bindet in WB, IP, IHC, Dot blot, CoIP, ELISA INSR und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human, Fisch, Hausschwein, Maus, Ratte, Zebrafisch |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | INSR fusion protein Ag13922 |
Vollständiger Name | INSR |
Berechnetes Molekulargewicht | 1382 aa, 156 kDa |
Beobachtetes Molekulargewicht | 190 kDa, 135 kDa, 95 kDa |
GenBank-Zugangsnummer | BC117172 |
Gene symbol | INSR |
Gene ID (NCBI) | 3643 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The biological effects of INS are mediated by a membrane spanning cell surface receptor that has been shown to consist of disulfide-linked 135-kDa and 95-kDa alpha- and beta-subunits that are cleaved products of a common precursor. Binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896). This antibody raised against 980-1382aa of human INS receptor can recognize full-length INS receptor precursor and INS receptor beta subunit.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for INSR antibody 20433-1-AP | Protokoll herunterladen |
IP protocol for INSR antibody 20433-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Environ Health Perspect Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice. | ||
Food Funct l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways | ||
Br J Surg MiR-128 regulation of glucose metabolism and cell proliferation in triple-negative breast cancer. | ||
FASEB J Angptl7 promotes insulin resistance and type 2 diabetes mellitus by multiple mechanisms including SOCS3-mediated IRS1 degradation. | ||
Sci Rep NaoXinTong Capsules inhibit the development of diabetic nephropathy in db/db mice. | ||
J Mol Med (Berl) Metabolomics reveals that chronic restraint stress alleviates carbon tetrachloride-induced hepatic fibrosis through the INSR/PI3K/AKT/AMPK pathway |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Mi (Verified Customer) (05-29-2023) | It works well in human white adipocytes, we get strong signals
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FH Chiara (Verified Customer) (10-04-2021) | Works well but three bands
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FH Boyan (Verified Customer) (06-06-2021) | This antibody recognized a band at ~ 180 kd only, which is sensitive to PNGaseF, but not EndoH, treatment. The size is much bigger than an expected InsR band. Importantly, stripping the membrane and re-blot with another InsR antibody from a different brand could detect the protein at the expected MW. These results suggest this InsR antibody is detecting something other than InsR.
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