INSIG2 Polyklonaler Antikörper

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• KD/KO Validated

INSIG2 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 24766-1-AP

Synonyme

INSIG 2, INSIG2

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Galerie der Validierungsdaten

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  WB analysis of rat liver using 24766-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 24766-1-AP (INSIG2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 24766-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 24766-1-AP (INSIG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 24766-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24766-1-AP (INSIG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 24766-1-AP

  IP Result of anti-INSIG2 (IP:24766-1-AP, 4ug; Detection:24766-1-AP 1:500) with mouse liver tissue lysate 3600ug.

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  IHC staining of human liver using 24766-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24766-1-AP (INSIG2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 24766-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24766-1-AP (INSIG2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 24766-1-AP

  Immunofluorescent analysis of HepG2 cells using 24766-1-AP (INSIG2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlebergewebe, A549-Zellen, SGC-7901-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

24766-1-AP bindet in WB, IP, IHC, IF, ELISA INSIG2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: INSIG2 fusion protein Ag14072
• Vollständiger Name: INSIG 2
• Berechnetes Molekulargewicht: 225 aa, 25 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC022475
• Gene symbol: INSIG2
• Gene ID (NCBI): 51141
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

INSIG2 mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. INSIG2 may bring HMGCR into ubiquitin-mediated proteasomal degradation. Insig2 protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. INSIG-2 may play an important role in therapy of hypercholesterolemia.

Publikationen

Species	Application	Title
human	WB,IP	Nature; The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.; Authors - Daqian Xu ; KD Validated
human	WB,IF	Mol Metab; CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.; Authors - Han Zeng ; KD Validated
mouse	WB,IP	Int J Biol Sci; The GR-gp78 Pathway is involved in Hepatic Lipid Accumulation Induced by Overexpression of 11β-HSD1.; Authors - Mengliang Hu
human	WB	Am J Cancer Res; p38α/S1P/SREBP2 activation by the SAM-competitive EZH2 inhibitor GSK343 limits its anticancer activity but creates a druggable vulnerability in hepatocellular carcinoma.; Authors - Pei-Ming Yang
human	WB	Proteomes; A Multi-Level Systems Biology Analysis of Aldrin's Metabolic Effects on Prostate Cancer Cells; Authors - Carmen Bedia ; KD Validated
mouse	WB	Nat Commun; In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion; Authors - Hasan Vatandaslar