INSIG1 Polyklonaler Antikörper

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• KD/KO Validated

INSIG1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 22115-1-AP

Synonyme

CL 6, INSIG 1, INSIG1, insulin induced gene 1, Insulin induced gene 1 protein

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Galerie der Validierungsdaten

• 

  WB analysis of A549 using 22115-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 22115-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 22115-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22115-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 22115-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HepG2-Zellen, MCF-7-Zellen, SGC-7901-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

22115-1-AP bindet in WB, IHC, IF, ELISA INSIG1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: INSIG1 fusion protein Ag17420
• Vollständiger Name: insulin induced gene 1
• Berechnetes Molekulargewicht: 277 aa, 30 kDa
• Beobachtetes Molekulargewicht: 28-31 kDa, 66-70 kDa
• GenBank-Zugangsnummer: BC001880
• Gene symbol: INSIG1
• Gene ID (NCBI): 3638
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Insulin-induced genes (INSIGs), which consist of two isoforms (INSIG1 and INSIG2), are representative endoplasmic reticulum (ER)-resident oxysterol-binding proteins (OSBPs). INSIG1 is part of negative regulatory feedback, acting as a brake on SREBP transcriptional function (PMID: 23919961). INSIG1 also has a secondary function mediating sterolaccelerated proteolytic degradation of HMG CoA reductase, thus impacting the mevalonate pathway. Suppression of INSIG1 function promotes hepatic lipid remodelling and restrains NASH (non-alcoholic steatohepatitis) progression(PMID: 33722690).

Publikationen

Species	Application	Title
human	WB,IF	Nature; The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.; Authors - Daqian Xu ; KD Validated
	WB	Transl Psychiatry; A potential mechanism underlying atypical antipsychotics-induced lipid disturbances.; Authors - H L Cai
	WB	Eur Neuropsychopharmacol; Vitamin D deficiency exacerbates atypical antipsychotic-induced metabolic side effects in rats: Involvement of the INSIG/SREBP pathway.; Authors - Ruili Dang
	WB	Mol Nutr Food Res; (-)-Epicatechin regulates blood lipids and attenuates hepatic steatosis in rats fed high-fat diet(†).; Authors - Hui Cheng
	WB,IHC	J Physiol Biochem; Renal tubule ectopic lipid deposition in diabetic kidney disease rat model and in vitro mechanism of leptin intervention.; Authors - Shasha Liu
human	IF	JCI Insight; Hypomorphic ASGR1 modulates lipid homeostasis via INSIG1-mediated SREBP signaling suppression.; Authors - Yingying Xu