INSC Polyklonaler Antikörper

INSC Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 20973-1-AP

Synonyme

INSC, Protein inscuteable homolog

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 20973-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20973-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20973-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20973-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 20973-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20973-1-AP (INSC antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 20973-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20973-1-AP (INSC antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 20973-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20973-1-AP (INSC antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 20973-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20973-1-AP (INSC antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 20973-1-AP

  Immunofluorescent analysis of HeLa cells using 20973-1-AP (INSC antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• ChIP: See 1 publications below

Produktinformation

20973-1-AP bindet in WB, IHC, IF, ChIP, ELISA INSC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: inscuteable homolog (Drosophila)
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: NM_001031853
• Gene symbol: INSC
• Gene ID (NCBI): 387755
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

INSC may function as an adapter linking the Par3 complex to the GPSM1/GPSM2 complex. It is involved in spindle orientation during mitosis it may regulate cell proliferation and differentiation in the developing nervous system. INSC may play a role in the asymmetric division of fibroblasts and participate in the process of stratification of the squamous epithelium. The antibody can recognize isoform1, 2,4,5 of INSC.

Publikationen

Species	Application	Title
	ChIP	Cell Rep; The transcription factor ZEB1 regulates stem cell self-renewal and cell fate in the adult hippocampus.; Authors - Bhavana Gupta