INPP5J Polyklonaler Antikörper

INPP5J Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 21417-1-AP

Synonyme

INPP5, INPP5J, PIB5PA, PIPP

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Galerie der Validierungsdaten

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  WB analysis of human heart using 21417-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21417-1-AP (INPP5J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 21417-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21417-1-AP (INPP5J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 21417-1-AP

  IP Result of anti-INPP5J (IP:21417-1-AP, 4ug; Detection:21417-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human kidney using 21417-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21417-1-AP (INPP5J antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 21417-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21417-1-AP (INPP5J antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 21417-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21417-1-AP (INPP5J antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 21417-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21417-1-AP (INPP5J antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 21417-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 21417-1-AP (INPP5J antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, Mauslungengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

21417-1-AP bindet in WB, IP, IHC, IF,ELISA INPP5J und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: INPP5J fusion protein Ag15996
• Vollständiger Name: inositol polyphosphate-5-phosphatase J
• Berechnetes Molekulargewicht: 1006 aa, 107 kDa
• Beobachtetes Molekulargewicht: 107 kDa, 70 kDa
• GenBank-Zugangsnummer: BC109288
• Gene symbol: INPP5J
• Gene ID (NCBI): 27124
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

INPP5J(Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A) is also named as PIB5PA, PIPP and belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family. INPP5J is a key signalling molecule that regulates dendritic outgrowth through activation of small GTPase signalling via interaction between FRMPD4 and ARHGEF7. It can control the resting membrane potential through a specific ion-channel-receptor-ligand interaction that brings about a large conformational change, analogous to neurotransmitter activation of ion channels at synapses. (PMID:22595022). This protein has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB,IHC	Nat Commun; miR-508 sustains phosphoinositide signalling and promotes aggressive phenotype of oesophageal squamous cell carcinoma.; Authors - Chuyong Lin
human	WB	Biomed Pharmacother; MiR-661 contributed to cell proliferation of human ovarian cancer cells by repressing INPP5J expression.; Authors - Tongyu Zhu