INO80B Polyklonaler Antikörper

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• KD/KO Validated

INO80B Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29488-1-AP

Synonyme

hIes2, HMGA1L4, HMGIYL4, IES2, INO80 complex subunit B, INO80B, PAP 1 associated protein 1, PAP 1BP, PAPA 1, PAPA1, ZNHIT4

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Galerie der Validierungsdaten

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  WB analysis using 29488-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 29488-1-AP (INO80B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 29488-1-AP

  WB result of INO80B antibody (29488-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INO80B transfected HeLa cells.

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  IHC staining of mouse ovary using 29488-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 29488-1-AP (INO80B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 29488-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 29488-1-AP (INO80B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 29488-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using INO80B antibody (29488-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Maus-Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29488-1-AP bindet in WB, IHC, IF, ELISA INO80B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: INO80B fusion protein Ag30345
• Vollständiger Name: INO80 complex subunit B
• Berechnetes Molekulargewicht: 356 aa, 39 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC050666
• Gene symbol: INO80B
• Gene ID (NCBI): 83444
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

INO80B (INO80 complex subunit B), also known as ZNHIT4 (zinc finger HIT domain-containing protein 4), PAPA1 (PAP-1-associated protein 1), is a subunit of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. INO80B is localized to the nucleolus. The function of INO80B is to induce growth arrest by haulting the cell cycle at the G1 phase. INO80B expression is controlled at the transcriptional level and is highest at the G0 and G1 phases of the cell cycle. In vitro, INO80B binds PAP-1, a splicing factor, and may play a role in nucleolar complexes that regulate ribosome biogenesis and cell cycle events.