ING3-specific Polyklonaler Antikörper

ING3-specific Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16187-1-AP

Synonyme

Eaf4, ING2, ING3, p47ING3

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Galerie der Validierungsdaten

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  WB analysis of human kidney using 16187-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16187-1-AP (ING3-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16187-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16187-1-AP (ING3-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16187-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16187-1-AP (ING3-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16187-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16187-1-AP (ING3-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 16187-1-AP

  IP Result of anti-ING3-specific (IP:16187-1-AP, 4ug; Detection:16187-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe, Mausherzgewebe, Mauslebergewebe, Mausnierengewebe
• Erfolgreiche IP: Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16187-1-AP bindet in WB, IP,ELISA ING3-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: inhibitor of growth family, member 3
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: NM_019071
• Gene symbol: ING3
• Gene ID (NCBI): 54556
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Members of inhibitor of growth (ING) family function in inhibiting cell growth and inducing apoptosis. They are sequence homologous proteins. ING3 can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. This antibody is specifically against p47ING3.